New cell lines from Ephestia kuehniella: Characterization and susceptibility to baculoviruses

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New cell lines from Ephestia kuehniella: characterization and susceptibility to baculoviruses

New cell lines from embryos of Ephestia kuehniella were recently developed. Primary cultures were initiated in September 2002 from 2 to 4 day old eggs in either modified TC-100 or ExCell 400 medium. From these initial cultures, one, originally isolated in the Ex-Cell medium, produced sufficient cell growth to allow subcultivation and eventually led to the establishment of two cell strains, one ...

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Many embryonic Pieris rapae (imported cabbageworm) cell lines were established from 24-hr-old P. rapae eggs. Some were characterized by growth curve analysis and by malate and lactate dehydrogenase isozyme comparison with other lepidopteran and mosquito cell lines. Most of the cell lines were screened for susceptibility to P. rapae granulosis virus using the peroxidase antiperoxidase assay and ...

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Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation

Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cu...

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Hypervariable Bkm DNA Loci in a Moth, Ephestia kuehniella : Does Transposition Cause Restriction Fragment Length Polymorphism?

Bkm sequences, originally isolated from snake satellite DNA, are a component of eukaryote genomes with a preferential location on sex chromosomes. In the Ephestia genome, owing to the presence of only a few Bkm-positive BamHI restriction fragments and to extensive restriction fragment length polymorphisms between and within inbred strains, a genetic crossbreeding analysis was feasible. No sex l...

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Developmental and Reproductive Biology of the Ectoparasitoid, Elasmus steffani, in a Substitute Host, Ephestia kuehniella

Elasmus steffani (Viggiani) (Hymenoptera: Elasmidae) is a gregarious idiobiont ectoparasitoid of Prays oleae (Bernard) (Lepidoptera: Plutellidae), an olive crop pest. In the substitute host Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae), the duration of the developmental stages was approximately 11-15 days. The preoviposition was 8.9 ± 5.0 days, and oviposition lasted 30.4 ± 10.5 days, w...

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ژورنال

عنوان ژورنال: Journal of Insect Science

سال: 2004

ISSN: 1536-2442

DOI: 10.1093/jis/4.1.9